6HQ9

Crystal structure of the Tudor domain of human ERCC6-L2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	23.539, 54.399, 108.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.410 - 1.982
R-factor	0.2255
Rwork	0.224
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fdr
RMSD bond length	0.003
RMSD bond angle	0.524
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.410	2.030
High resolution limit [Å]	1.980	1.980
Rmerge	0.193	4.040
Rpim	0.035	0.710
Number of reflections	10359	719
<I/σ(I)>	12.8	2.1
Completeness [%]	100.0	100
Redundancy	31.5	32.8
CC(1/2)	0.999	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Ammonium sulphate, 0.1 M Acetate pH 4.5, 18% PEG 4000