6HQ6
Bacterial beta-1,3-oligosaccharide phosphorylase from GH149
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.239, 158.986, 181.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.490 - 2.050 |
| R-factor | 0.1834 |
| Rwork | 0.182 |
| R-free | 0.21090 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.234 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.490 | 79.490 | 2.090 |
| High resolution limit [Å] | 2.050 | 11.230 | 2.050 |
| Rmerge | 0.107 | 0.027 | 2.387 |
| Rmeas | 0.111 | 0.028 | 2.478 |
| Rpim | 0.030 | 0.008 | 0.661 |
| Total number of observations | 2447545 | ||
| Number of reflections | 181781 | 1258 | 8926 |
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 13.5 | 11.8 | 13.8 |
| CC(1/2) | 0.999 | 0.999 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | Null |
