6HPV

Crystal structure of mouse fetuin-B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.7712
Spacegroup name	P 41 21 2
Unit cell lengths	67.710, 67.710, 197.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.878 - 2.300
R-factor	0.2255
Rwork	0.224
R-free	0.25290
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.586
Data reduction software	XDS (Jan 26, 2018 BUILT=20180409)
Data scaling software	XSCALE (Jan 26, 2018 BUILT=20180409)
Phasing software	CRANK2 (2.0.166)
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.109	1.626
Rmeas	0.112	1.782
Rpim	0.024	0.710
Number of reflections	19993	1303
<I/σ(I)>	20.34	1.36
Completeness [%]	93.6	63
Redundancy	19.3	6
CC(1/2)	0.999	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	150 mM NaCl, 100 mM NaOAc, 20 mM Na-HEPES pH 7.8, 25% PEG 4000, 8% isopropanol