6HPN
Crystal structure of Borrelia spielmanii WP_012665240 (BSA64) - an orthologous protein to B. burgdorferi BBA64
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.442, 77.442, 104.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.250 - 2.600 |
| R-factor | 0.2094 |
| Rwork | 0.208 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aly |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.612 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.250 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.105 | 0.280 |
| Number of reflections | 10298 | 1470 |
| <I/σ(I)> | 19 | 9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.2 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 294 | 0.1 M Sodium citrate 15% 2-propanol 18% PEG 4000 |
