6HPF
Structure of Inactive E165Q mutant of fungal non-CBM carrying GH26 endo-b-mannanase from Yunnania penicillata in complex with alpha-62-61-di-galactosyl-mannotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.92 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.988, 98.988, 170.497 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.240 - 1.360 |
R-factor | 0.12304 |
Rwork | 0.122 |
R-free | 0.14345 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zm8 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.095 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.240 | 1.380 |
High resolution limit [Å] | 1.360 | 1.360 |
Number of reflections | 105928 | |
<I/σ(I)> | 20.7 | |
Completeness [%] | 100.0 | |
Redundancy | 18.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulphate, Hepes pH 7.0. |