6HPD
The structure of a beta-glucuronidase from glycoside hydrolase family 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.929, 67.170, 96.720 |
| Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
| Resolution | 96.200 - 2.430 |
| R-factor | 0.16704 |
| Rwork | 0.165 |
| R-free | 0.21149 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dmy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.162 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.200 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.064 | 0.161 |
| Number of reflections | 37999 | 3961 |
| <I/σ(I)> | 11.3 | 5.8 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 10 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Sodium Bromide, 0.1M BIS-TRIS pH 7.5, 20% PEG 3350 |
