6HPC

Crystal structure of the HicB antitoxin from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	43.090, 60.380, 59.340
Unit cell angles	90.00, 103.76, 90.00

Refinement procedure

Resolution	57.640 - 2.260
Rwork	0.227
R-free	0.26790
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.640	2.341
High resolution limit [Å]	2.260	2.260
Rmerge	0.033	0.108
Number of reflections	13836	1363
<I/σ(I)>	1.18	1.18
Completeness [%]	98.8	98.62
Redundancy	3.4	1.9
CC(1/2)	0.999	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	4 % Tacsimate 12 % PEG 3350