6HOX
Crystal structure of the binding domain of Paraclostridial Mosquitocidal Protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.984 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.146, 38.686, 108.514 |
Unit cell angles | 90.00, 116.74, 90.00 |
Refinement procedure
Resolution | 48.460 - 1.950 |
R-factor | 0.17756 |
Rwork | 0.176 |
R-free | 0.20930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kbb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.206 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.460 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.097 | 0.725 |
Rmeas | 0.135 | 1.009 |
Rpim | 0.093 | 0.700 |
Number of reflections | 31355 | 2098 |
<I/σ(I)> | 8.3 | 1.6 |
Completeness [%] | 96.9 | 94.4 |
Redundancy | 3.5 | 3.3 |
CC(1/2) | 0.995 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 0.12 M Ethylene glycols, 0.1 M Buffer System 3 pH 8.5, 50 % v/v Precipitant Mix 4 (Morpheus screen, Molecular Dimensions) |