6HOM

Drosophila NOT4 CBM peptide bound to human CAF40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	83.920, 109.640, 69.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.160 - 2.100
R-factor	0.1926
Rwork	0.191
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fv2 chain A
RMSD bond length	0.002
RMSD bond angle	0.510
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	38143	2780
<I/σ(I)>	12.2	1.5
Completeness [%]	99.6	99.8
Redundancy	4	4.2
CC(1/2)	0.990	0.556

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	0.1 M Bis-Tris/Cl, pH 5.5, 0.2 M ammonium sulfate, 25 % (w/v) PEG 3350