6HOL

Structure of ATG14 LIR motif bound to GABARAPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	34.460, 41.620, 46.240
Unit cell angles	86.08, 76.27, 86.70

Refinement procedure

Resolution	41.485 - 1.400
R-factor	0.1655
Rwork	0.164
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r2q
RMSD bond length	0.015
RMSD bond angle	1.347
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.485	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.070	0.710
Rmeas	0.100	0.900
Rpim	0.060	0.550
Number of reflections	45978	4496
<I/σ(I)>	6.2	1.3
Completeness [%]	93.3	90.33
Redundancy	2.4	2.4
CC(1/2)	0.990	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	1M LiCl, 0.1M HEPES pH7, 20% PEG 6000