6HOJ
Structure of Beclin1 LIR motif bound to GABARAP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2015-09-21 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 76.670, 150.490, 35.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.703 - 1.510 |
R-factor | 0.1706 |
Rwork | 0.169 |
R-free | 0.21120 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.221 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.703 | 1.560 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmerge | 0.060 | 1.020 |
Rmeas | 0.070 | 1.180 |
Rpim | 0.030 | 0.570 |
Number of reflections | 65391 | 6405 |
<I/σ(I)> | 13.2 | 1.2 |
Completeness [%] | 99.5 | 98.89 |
Redundancy | 5.2 | 3.8 |
CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 15% PEG 4000, 50 mM Bicine pH 8.8 |