6HOG

Structure of VPS34 LIR motif bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	H 3
Unit cell lengths	92.100, 92.100, 41.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.600 - 1.260
R-factor	0.1556
Rwork	0.154
R-free	0.18090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.005
RMSD bond angle	0.793
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.600	1.300
High resolution limit [Å]	1.260	1.260
Rmerge	0.020	0.730
Rmeas	0.030	0.890
Rpim	0.010	0.490
Number of reflections	34959	10172
<I/σ(I)>	19.2	1.5
Completeness [%]	99.2	98.19
Redundancy	3.4	2.9
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM HEPES pH 7.1, 40% PEG 600