6HN7

Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	60.523, 101.004, 82.627
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.500 - 3.000
R-factor	0.3143
Rwork	0.312
R-free	0.33210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hlk 1j9i
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.500	50.500	3.180
High resolution limit [Å]	3.000	9.000	3.000
Rmerge	0.064	0.044	0.105
Rmeas	0.076	0.054	0.125
Rpim	0.041	0.030	0.066
Total number of observations	18007	659	3017
Number of reflections	5163	218	832
<I/σ(I)>	11.1	15.5	6.7
Completeness [%]	97.7	95.3	98.7
Redundancy	3.5	3	3.6
CC(1/2)	0.992	0.991	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	288	2M Ammonium Sulfate, 0.1M AcONa pH 5