6HMF
D-family DNA polymerase - DP1 subunit (3'-5' proof-reading exonuclease) H451 proof-reading deficient variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0427 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.750, 91.240, 143.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.190 - 2.600 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ihe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.647 | |
| Number of reflections | 35506 | 4266 |
| <I/σ(I)> | 12 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8.8 |
| CC(1/2) | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 277 | cacodylate, sodium chloride, PEG 8000, calcium acetate |
