6HM5
Crystal structure of TOPBP1 BRCT0,1,2 in complex with a RAD9 phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91407 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.050, 34.070, 66.670 |
Unit cell angles | 90.00, 103.57, 90.00 |
Refinement procedure
Resolution | 38.699 - 2.330 |
R-factor | 0.205336955188 |
Rwork | 0.201 |
R-free | 0.24149 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.516 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 2.413 |
High resolution limit [Å] | 2.330 | 2.330 |
Number of reflections | 11242 | |
<I/σ(I)> | 9.72 | |
Completeness [%] | 99.0 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 287.15 | 100 mM bicine/Trizma base pH 8.5, 300 mM diethyleneglycol, 300 mM triethyleneglycol, 300 mM tetraethyleneglycol, 300 mM pentaethyleneglycol, 10% w/v PEG 20000 and 20% v/v PEG MME 550 |