6HM4

Crystal structure of Rad4 BRCT1,2 in complex with a Mdb1 phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	50.170, 40.000, 54.960
Unit cell angles	90.00, 105.16, 90.00

Refinement procedure

Resolution	31.938 - 1.770
R-factor	0.16857933455
Rwork	0.166
R-free	0.21141
RMSD bond length	0.006
RMSD bond angle	0.833
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.940	1.833
High resolution limit [Å]	1.770	1.770
Number of reflections	19339
<I/σ(I)>	16.52
Completeness [%]	93.1
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287.15	200 mM Sodium acetate trihydrate, 100 mM Tris 8.5 and 30 % w/v PEG 4000