6HLK

Hijacking the Hijackers: Escherichia coli Pathogenicity Islands Redirect Helper Phage Packaging for Their Own Benefit.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 43 21 2
Unit cell lengths	56.881, 56.881, 132.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.120 - 2.420
R-factor	0.2388
Rwork	0.237
R-free	0.26710
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.679
Data scaling software	Aimless (0.5.32)
Phasing software	AutoSol (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.132	7.375
High resolution limit [Å]	2.416	2.416
Rmerge	0.073
Rpim	0.033
Number of reflections	7721
<I/σ(I)>	13.6
Completeness [%]	89.7
Redundancy	6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	288	15% PEG 8000, 0.1M Sodium Acetate pH6