6HLI
wild-type NuoEF from Aquifex aeolicus - reduced form bound to NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-07-22 |
Detector | MARRESEARCH |
Wavelength(s) | 1.541790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.590, 116.040, 189.875 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.300 - 2.380 |
R-factor | 0.206 |
Rwork | 0.205 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.000 | 2.510 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.119 | 0.757 |
Rpim | 0.051 | 0.351 |
Number of reflections | 57523 | 8284 |
<I/σ(I)> | 13.2 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.4 | 6.4 |
CC(1/2) | 0.998 | 0.778 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.1M Tris/HCl 0.1M Sodium citrate |