6HLA
wild-type NuoEF from Aquifex aeolicus - reduced form bound to NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.642, 116.431, 189.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.200 - 1.900 |
| R-factor | 0.2159 |
| Rwork | 0.215 |
| R-free | 0.24050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INHOUSE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless ((version 0.5.14)) |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 189.450 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.143 | 0.743 |
| Number of reflections | 110071 | 4524 |
| <I/σ(I)> | 9.5 | 2.43 |
| Completeness [%] | 98.7 | 83.7 |
| Redundancy | 7 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.1M Tris/HCl 0.1M Sodium citrate |
