6HL5

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP1(932-954)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	86.198, 86.198, 147.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.310 - 1.980
R-factor	0.1829
Rwork	0.182
R-free	0.20130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.014
RMSD bond angle	1.150
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.310	2.030
High resolution limit [Å]	1.980	1.980
Rmerge	0.080	2.350
Number of reflections	39456	2880
<I/σ(I)>	17	1.4
Completeness [%]	100.0	100
Redundancy	18.9	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes pH = 7.5, 1.2 M ammonium sulfate, 4.5% PEG400