6HL4
wild-type NuoEF from Aquifex aeolicus - reduced form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-10-25 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.741, 108.117, 188.181 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.960 - 2.060 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IN-HOUSE |
RMSD bond length | 0.008 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | AutoPROC |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 188.180 | 2.180 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.110 | 0.312 |
Rmeas | 0.135 | 0.420 |
Rpim | 0.060 | 0.242 |
Number of reflections | 65727 | 7715 |
<I/σ(I)> | 9.3 | 2.7 |
Completeness [%] | 86.7 | 71.1 |
Redundancy | 4.9 | 3.1 |
CC(1/2) | 0.994 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.1M Tris/HCl 0.1M Sodium citrate |