6HL3
wild-type NuoEF from Aquifex aeolicus - oxidized form bound to NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-01-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54790 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.514, 115.759, 189.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.350 - 2.040 |
| R-factor | 0.1746 |
| Rwork | 0.173 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless ((version 0.5.14)) |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.350 | 2.150 |
| High resolution limit [Å] | 2.036 | 2.036 |
| Rmerge | 0.123 | 0.904 |
| Number of reflections | 88168 | |
| <I/σ(I)> | 14.4 | 2.3 |
| Completeness [%] | 97.6 | 95.6 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.1 M Tris/HCl 0.1 M Sodium citrate |
