6HL2
wild-type NuoEF from Aquifex aeolicus - oxidized form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X12 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.653080 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.190, 116.438, 189.449 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.480 - 1.950 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19500 |
Structure solution method | SAD |
Starting model (for MR) | INHOUSE |
RMSD bond length | 0.009 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | Aimless ((version 0.5.12)) |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.440 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.162 | 1.561 |
Number of reflections | 102591 | |
<I/σ(I)> | 12.9 | 1.5 |
Completeness [%] | 99.9 | 97.8 |
Redundancy | 15.9 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 292 | 0.1 M Tris/HCl, 1.0 M sodium citrate |