6HKT

Structure of an H1-bound 6-nucleosome array

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.991870
Spacegroup name	P 2 21 21
Unit cell lengths	111.078, 238.761, 674.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.074 - 9.700
R-factor	0.2554
Rwork	0.254
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lz0
RMSD bond length	0.004
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	10.850
High resolution limit [Å]	9.700	9.700
Rmerge	0.211	0.881
Rmeas	0.230
Rpim	0.090	0.362
Number of reflections	11252	3152
<I/σ(I)>	7	2.3
Completeness [%]	99.2	100
Redundancy	6.4	6.8
CC(1/2)	0.998	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293.15	15% MPD, 0.1 M NaCl and 0.1 M Tris pH 8.8