6HKR
Human Cellular Retinoic Acid Binding Protein II (CRABPII) with bound synthetic retinoid DC271.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.689, 47.479, 77.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.550 - 1.500 |
R-factor | 0.14462 |
Rwork | 0.143 |
R-free | 0.17548 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fr3 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.903 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.550 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 27170 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 100.0 | |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG5000, TRIS HCL, Sodium Acetate |