6HKE
MatC (Rpa3494) from Rhodopseudomonas palustris with bound malate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.800, 205.750, 47.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.540 - 2.110 |
R-factor | 0.19328 |
Rwork | 0.191 |
R-free | 0.24814 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oei |
RMSD bond length | 0.007 |
RMSD bond angle | 1.548 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.540 | 2.170 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.046 | 0.591 |
Number of reflections | 41492 | 2471 |
<I/σ(I)> | 12.4 | 1.6 |
Completeness [%] | 88.9 | 65.3 |
Redundancy | 1.8 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 290 | Tris 0.1 M pH 8.5, MgCl2 0.2 M, PEG 8000 20% (w/v) |