6HK5
X-ray structure of a truncated mutant of the metallochaperone CooJ with a high-affinity nickel-binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.800, 74.280, 70.640 |
| Unit cell angles | 90.00, 92.74, 90.00 |
Refinement procedure
| Resolution | 46.746 - 2.042 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.25850 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.746 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmeas | 0.140 | 0.880 |
| Number of reflections | 30586 | 4749 |
| <I/σ(I)> | 9.4 | 1.63 |
| Completeness [%] | 99.2 | 96 |
| Redundancy | 6.4 | 6.2 |
| CC(1/2) | 0.997 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | protein concentration of 10 mgmL-1 and 1 equivalent of Ni(II) per dimer, crystals were grown in 16% PEG3350 and 0.2 M calcium chloride |
