6HJW

Crystal structure of the chloroplast chorismate mutase from Zea mays

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.87260
Spacegroup name	C 2 2 21
Unit cell lengths	61.800, 89.830, 216.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.086 - 2.500
R-factor	0.2252
Rwork	0.223
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3csm
RMSD bond length	0.003
RMSD bond angle	0.578
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.083	1.018
Number of reflections	21328	2101
<I/σ(I)>	12.46	1.56
Completeness [%]	99.5	99.48
Redundancy	6.6	6.3
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Ammonium tartrate, 20 % PEG 3350