6HJW
Crystal structure of the chloroplast chorismate mutase from Zea mays
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.87260 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.800, 89.830, 216.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.086 - 2.500 |
R-factor | 0.2252 |
Rwork | 0.223 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3csm |
RMSD bond length | 0.003 |
RMSD bond angle | 0.578 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.920 | 2.580 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.083 | 1.018 |
Number of reflections | 21328 | 2101 |
<I/σ(I)> | 12.46 | 1.56 |
Completeness [%] | 99.5 | 99.48 |
Redundancy | 6.6 | 6.3 |
CC(1/2) | 0.990 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M Ammonium tartrate, 20 % PEG 3350 |