6HJU
The X-ray structure of the horse spleen ferritin nanocage containing Pt, obtained upon encapsulation of a Pt(II) terpyridine compound within the protein cage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.54 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 180.530, 180.530, 180.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.300 - 1.580 |
R-factor | 0.1342 |
Rwork | 0.132 |
R-free | 0.18214 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.163 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BUSTER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.300 | 1.630 |
High resolution limit [Å] | 1.580 | 1.580 |
Rpim | 0.569 | |
Number of reflections | 33571 | |
<I/σ(I)> | 15.7 | |
Completeness [%] | 95.9 | |
Redundancy | 44.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.6 M (NH4)2SO4, 0.1 M Tris HCl pH 7.4, 60 mM CdSO4 |