6HJO

Myxococcus xanthus MglA bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978570
Spacegroup name	P 21 21 21
Unit cell lengths	53.980, 89.180, 118.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.130 - 1.980
R-factor	0.227
Rwork	0.227
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3t1o
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.130	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.121	1.280
Rmeas	0.136	1.276
Number of reflections	40545	3634
<I/σ(I)>	13.07	0.6
Completeness [%]	98.0	100
Redundancy	4.4	4.1
CC(1/2)	0.994	0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium cacodylate pH 6.5, 40% MPD and PEG 8 000