6HJK
Crystal Structure of Aurora-A L210C catalytic domain in complex with ASDO2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92818 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 88.769, 88.769, 77.280 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.876 - 2.400 |
R-factor | 0.2189 |
Rwork | 0.217 |
R-free | 0.25230 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.221 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.880 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 14112 | 1373 |
<I/σ(I)> | 23.07 | 4.63 |
Completeness [%] | 100.0 | 100 |
Redundancy | 2 | 2 |
CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M tri-sodium citrate pH 5.6, 25 % w/v polyethylene glycol 4000 |