6HJI
Xray structure of GLIC in complex with crotonate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2016-07-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.943, 133.242, 159.880 |
Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.800 |
R-factor | 0.1896 |
Rwork | 0.189 |
R-free | 0.20430 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 49.000 |
High resolution limit [Å] | 2.800 | 2.900 |
Number of reflections | 91038 | |
<I/σ(I)> | 9.2 | |
Completeness [%] | 99.5 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM Na-crotonate pH4 400 mM Na-Isothiocyanate 12-15% PEG4000 2% DMSO 15% glycerol |