6HJH
Myxococcus xanthus MglA bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.550, 73.550, 97.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.600 - 3.300 |
| R-factor | 0.197 |
| Rwork | 0.191 |
| R-free | 0.31600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t1o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.230 | 1.600 |
| Rmeas | 0.260 | 1.800 |
| Number of reflections | 4841 | 135 |
| <I/σ(I)> | 7.26 | |
| Completeness [%] | 47.0 | 100 |
| Redundancy | 5.2 | 5.4 |
| CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES 0.1M, PEG 4000 30% |
