6HJ5

Crystal structure of Whitewater Arroyo virus GP1 glycoprotein at pH 5.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 63 2 2
Unit cell lengths	109.040, 109.040, 70.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.520 - 2.080
R-factor	0.1786
Rwork	0.177
R-free	0.20700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.860
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.130
High resolution limit [Å]	2.080	2.080
Rmerge	0.132	2.213
Number of reflections	15428
<I/σ(I)>	18
Completeness [%]	100.0
Redundancy	18.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	10.1 mg/ml protein, 0.2 M potassium sodium tartrate, 2 M ammonium sulphate and 0.1 M tri-sodium citrate pH 5.6