6HJ5
Crystal structure of Whitewater Arroyo virus GP1 glycoprotein at pH 5.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-19 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97950 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 109.040, 109.040, 70.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.520 - 2.080 |
R-factor | 0.1786 |
Rwork | 0.177 |
R-free | 0.20700 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.860 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.130 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.132 | 2.213 |
Number of reflections | 15428 | |
<I/σ(I)> | 18 | |
Completeness [%] | 100.0 | |
Redundancy | 18.8 | |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 10.1 mg/ml protein, 0.2 M potassium sodium tartrate, 2 M ammonium sulphate and 0.1 M tri-sodium citrate pH 5.6 |