6HHN

Crystal structure of L-rhamnose mutarotase FA22100 from Formosa agariphila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979140
Spacegroup name	P 43 21 2
Unit cell lengths	70.913, 70.913, 54.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.166 - 1.470
R-factor	0.1491
Rwork	0.148
R-free	0.17210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qlx
RMSD bond length	0.008
RMSD bond angle	0.889
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.170	1.550
High resolution limit [Å]	1.470	1.470
Rmerge	0.032	0.295
Rmeas	0.034	0.315
Rpim	0.012	0.107
Number of reflections	24225	3383
<I/σ(I)>	32.3	6.6
Completeness [%]	99.5	97.2
Redundancy	9.1	8.5
CC(1/2)	0.999	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Sodium acetate pH4.6 4.3 M Sodium formate