6HGB
Influenza A virus N6 neuraminidase native structure (Duck/England/56).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-12-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.93 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.200, 73.900, 106.400 |
Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
Resolution | 30.680 - 1.500 |
R-factor | 0.14017 |
Rwork | 0.139 |
R-free | 0.16136 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v0z |
RMSD bond length | 0.013 |
RMSD bond angle | 1.706 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.680 | 1.520 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 241948 | 10672 |
<I/σ(I)> | 9.5 | 2.7 |
Completeness [%] | 92.1 | 82.5 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | N6 crystals were grown by hanging-drop vapour diffusion against 1:1 20%(w/v) PEG-3350 and 2 microM CaCl2 in 150mM NaCl at 20 degrees celsius, starting with equal volumes of N6 (20mg/ml in saline) and 20% (w/v) PEG 3350 and 2 microM in 1% saline. Microseeding with crystals of native N6 was carried out using a super saturated solution of N6 NA in 20% PEG 3350. |