Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

6HGB

Influenza A virus N6 neuraminidase native structure (Duck/England/56).

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-1
Synchrotron siteSSRL
BeamlineBL9-1
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1999-12-06
DetectorMAR scanner 345 mm plate
Wavelength(s)0.93
Spacegroup nameP 1 21 1
Unit cell lengths106.200, 73.900, 106.400
Unit cell angles90.00, 90.30, 90.00
Refinement procedure
Resolution30.680 - 1.500
R-factor0.14017
Rwork0.139
R-free0.16136
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1v0z
RMSD bond length0.013
RMSD bond angle1.706
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareREFMAC
Refinement softwareREFMAC (5.8.0230)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.6801.520
High resolution limit [Å]1.5001.500
Number of reflections24194810672
<I/σ(I)>9.52.7
Completeness [%]92.182.5
Redundancy3.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP293N6 crystals were grown by hanging-drop vapour diffusion against 1:1 20%(w/v) PEG-3350 and 2 microM CaCl2 in 150mM NaCl at 20 degrees celsius, starting with equal volumes of N6 (20mg/ml in saline) and 20% (w/v) PEG 3350 and 2 microM in 1% saline. Microseeding with crystals of native N6 was carried out using a super saturated solution of N6 NA in 20% PEG 3350.

224572

PDB entries from 2024-09-04

PDB statisticsPDBj update infoContact PDBjnumon