6HG0
Influenza A Virus N9 Neuraminidase complex with NANA (Tern/Australia).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 181.200, 181.200, 181.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.470 - 1.300 |
| R-factor | 0.0927 |
| Rwork | 0.091 |
| R-free | 0.11993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nn9 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.082 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.470 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 122385 | 6028 |
| <I/σ(I)> | 16.7 | 4.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 14 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | N9 crystals were grown by hanging-drop vapour diffusion against a reservoir of 1.9M potassium phosphate, pH 6.8, starting with equal volumes of N9 NA (10-15 mg/ml in water) and potassium phosphate buffer 1.4M KH2PO4:3M K2HPO4 in ratio 8:4, pH 6.6 at 20 degrees celsius. Inhibitor complexes obtained by soaking N9 crystals in a solution of 1.4M potassium phosphate buffer, pH 6.8, containing 5mM of inhibitor for 3 hours at 4 degrees celsius. Soaked in glycerol cryo-buffer. |
