6HFY
Influenza A virus N6 neuraminidase complex with DANA (Duck/England/56).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-11-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.995, 75.763, 105.976 |
Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
Resolution | 20.050 - 1.650 |
R-factor | 0.1571 |
Rwork | 0.156 |
R-free | 0.18463 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v0z |
RMSD bond length | 0.016 |
RMSD bond angle | 1.768 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.050 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 184006 | 8244 |
<I/σ(I)> | 8.1 | 2.4 |
Completeness [%] | 91.4 | 83.2 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | N6 crystals were grown by hanging-drop vapour diffusion against 1:1 20%(w/v) PEG-3350 and 2 microM CaCl2 in 150mM NaCl at 20 degrees celsius, starting with equal volumes of N6 (20mg/ml in saline) and 20% (w/v) PEG 3350 and 2 microM in 1% saline. Microseeding with crystals of native N6 was carried out using a super saturated solution of N6 NA in 20% PEG 3350. Inhibitor complex obtained by soaking N6 crystals in a solution of 20% PEG 3350 in 1% saline (mother liquor) containing 27mM DANA at pH7 for 3 hours at 18 degrees celsius. |