6HFT
Hsp90 co-chaperone Cns1 from Saccharomyces cerevisiae (delta69)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97793 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 50.780, 50.780, 283.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.26084 |
| Rwork | 0.259 |
| R-free | 0.28805 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hfm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.083 | 0.613 |
| Number of reflections | 9753 | |
| <I/σ(I)> | 10.6 | 2.2 |
| Completeness [%] | 97.9 | 99.8 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris/HCl pH 8.5, 25% PEG3000 |
