6HFM
Hsp90 co-chaperone Cns1 C-domain from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.000, 79.000, 98.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.550 |
R-factor | 0.16173 |
Rwork | 0.160 |
R-free | 0.18635 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.205 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.650 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.045 | 0.536 |
Number of reflections | 51808 | |
<I/σ(I)> | 18.1 | 3.4 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES pH 6.5, 25% PEG3000 |