6HFG
Structure of the REC114 PH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-29 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96600 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.490, 107.490, 82.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.590 - 2.500 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28870 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.057 | |
| Number of reflections | 10262 | 749 |
| <I/σ(I)> | 24.2 | 1.1 |
| Completeness [%] | 99.6 | 97.5 |
| Redundancy | 11.2 | 11.1 |
| CC(1/2) | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.25 M ammonium sulphate, 0.1 M MES (pH 6.5), 28% PEG 5000 MME |
