6HFC
Influenza A Virus N9 Neuraminidase Native (Tern).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.83 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 180.000, 180.000, 180.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.890 - 1.290 |
| R-factor | 0.14238 |
| Rwork | 0.141 |
| R-free | 0.16367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nn9 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.037 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.890 | 1.320 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Number of reflections | 122339 | 5993 |
| <I/σ(I)> | 11.7 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.5 | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | N9 crystals were grown by-hanging drop vapour diffusion against a reservoir of 1.9M potassium phosphate, pH 6.8, starting with equal volumes of N9 NA solution (10 to 15 mg/ml in water) and potassium phosphate buffer 1.4M KH2PO4: 3M K2HPO4 in ratio 8:4, pH 6.6 at 20 degrees celsius. Large rhombic dodecahedral crystals grew within a few days. |
