6HF2

The structure of BoMan26B, a GH26 beta-mannanase from Bacteroides ovatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	66.812, 68.376, 79.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.787 - 1.690
R-factor	0.169
Rwork	0.167
R-free	0.20630
RMSD bond length	0.007
RMSD bond angle	0.863
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.790	1.750
High resolution limit [Å]	1.690	1.690
Number of reflections	40664
<I/σ(I)>	18.23
Completeness [%]	98.5
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	0.1 M bis-tris, 20% PEG4000, 0.2 M NaCl, 0.3 mM CaCl2