6HEQ
Prion nanobody 484
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.040, 37.150, 83.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.850 - 1.230 |
| R-factor | 0.20009 |
| Rwork | 0.199 |
| R-free | 0.22398 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.307 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.850 | 1.258 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Number of reflections | 26127 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 98.2 | |
| Redundancy | 4.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG4K, 0.2M MgCl2, 0.1M Tris pH 8.5 |
