6HEQ
Prion nanobody 484
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.040, 37.150, 83.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.850 - 1.230 |
R-factor | 0.20009 |
Rwork | 0.199 |
R-free | 0.22398 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.307 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.850 | 1.258 |
High resolution limit [Å] | 1.230 | 1.230 |
Number of reflections | 26127 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 98.2 | |
Redundancy | 4.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG4K, 0.2M MgCl2, 0.1M Tris pH 8.5 |