6HEL

Structure of human USP25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9790
Spacegroup name	I 4
Unit cell lengths	139.184, 139.184, 190.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.166 - 2.941
R-factor	0.2551
Rwork	0.254
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6HEH and 6HEK
RMSD bond length	0.018
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.170	3.390
High resolution limit [Å]	2.940	2.940
Rmerge	0.052	1.430
Rmeas	0.058	1.570
Number of reflections	20988	1050
<I/σ(I)>	15.9	1.2
Completeness [%]	93.4	70.1
Redundancy	5.3	5.9
CC(1/2)	0.999	0.501

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	12% (w/v) PEG 3350, 167 mM magnesium acetate