6HEJ

Structure of human USP28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	104.235, 200.455, 206.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.842 - 2.790
R-factor	0.2619
Rwork	0.261
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6heh
RMSD bond length	0.005
RMSD bond angle	0.773
Data reduction software	xia2
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	143.680	3.030
High resolution limit [Å]	2.790	2.790
Rmerge	0.053	1.030
Rmeas	0.059	1.160
Number of reflections	31781	1590
<I/σ(I)>	15.6	1.5
Completeness [%]	93.5	62.7
Redundancy	5.1	4.6
CC(1/2)	0.998	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.8 M ammonium sulfate, 100 mM sodium citrate pH 5.5