6HDT
crystal structure of short afifavidin - biotin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.131, 65.218, 67.738 |
| Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
| Resolution | 46.620 - 1.540 |
| R-factor | 0.16571 |
| Rwork | 0.163 |
| R-free | 0.22012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hds |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.645 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.620 | 1.590 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 52210 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 95.8 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1.3-1.5M Ammonium Sulfate, 0.1M Acetate pH 5.0 |
