6HDI
R49A variant of beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 2.0 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.200, 116.900, 53.170 |
| Unit cell angles | 90.00, 98.09, 90.00 |
Refinement procedure
| Resolution | 52.640 - 2.030 |
| R-factor | 0.20879 |
| Rwork | 0.205 |
| R-free | 0.27283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.505 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.640 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.131 | 0.829 |
| Rmeas | 0.153 | 0.965 |
| Rpim | 0.077 | 0.488 |
| Number of reflections | 29676 | 1446 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 3.7 | 3.8 |
| CC(1/2) | 0.992 | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000 200mM sodium acetate 50mM TRIS pH 7.5 |
