6HDH
R49K variant of beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 1.6 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.130, 117.230, 53.010 |
| Unit cell angles | 90.00, 97.41, 90.00 |
Refinement procedure
| Resolution | 31.360 - 1.620 |
| R-factor | 0.18379 |
| Rwork | 0.182 |
| R-free | 0.21753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whe |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.501 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.360 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.052 | 0.770 |
| Rmeas | 0.060 | 0.917 |
| Rpim | 0.030 | 0.487 |
| Number of reflections | 56242 | 2748 |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 96.1 | 92.7 |
| Redundancy | 3.7 | 3.4 |
| CC(1/2) | 0.998 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000 200mM sodium acetate 50mM TRIS pH 7.5 |
