6HDF
D170N variant of beta-phosphoglucomutase from Lactococcus lactis in an open conformer to 1.4 A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.770, 119.310, 53.170 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 52.980 - 1.400 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.22352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.502 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.980 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.091 | 1.243 |
| Rmeas | 0.107 | 1.431 |
| Rpim | 0.055 | 0.701 |
| Number of reflections | 90031 | 4464 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 95.4 | 95.1 |
| Redundancy | 3.8 | 4 |
| CC(1/2) | 0.997 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 291 | 24-34% PEG 4000 200mM sodium acetate 50mM TRIS pH 7.5 |
